Search found 5 matches

by traini82
Wed Jul 07, 2010 9:36 am
Forum: Support & Questions
Topic: Align to Reference Sequence
Replies: 8
Views: 66119

Re: Align to Reference Sequence

Thanks for the links, is there a way to use "Align in Groups" to assemble to a reference sequence (i.e. Sequencher's "To Reference by Name")
by traini82
Thu Jun 17, 2010 10:34 am
Forum: Support & Questions
Topic: Align to Reference Sequence
Replies: 8
Views: 66119

Re: Align to Reference Sequence

I believe the new Reference Shortening feature in v 3.5 which allows automatic clipping of very long reference sequences to the region you want to look at addresses the multi-exon support previously discussed in this thread? I'd like to try it but unfortunately there are no notes where to find it or...
by traini82
Fri Nov 14, 2008 4:41 pm
Forum: Support & Questions
Topic: Align to Reference Sequence
Replies: 8
Views: 66119

Re: Align to Reference Sequence

Sorry to hear that the exon feature will not appear in the next build but at least its on the list. It would certainly be ok to contact me with questions about this and any other proposed feature. I'm not sure why emails were not received if sent to the address provided at registration. I haven't no...
by traini82
Thu Nov 13, 2008 12:35 pm
Forum: Support & Questions
Topic: Align to Reference Sequence
Replies: 8
Views: 66119

Re: Align to Reference Sequence

Could I make a request to add this to the 'wish list' as such a function would also be extremely useful in cases where we are doing SNP discovery in cell line cDNA. We could use genomic reference which has known SNPs annotated in it. It would be the reverse of Sequenchers ability to insert large gap...
by traini82
Mon Nov 10, 2008 4:09 pm
Forum: Support & Questions
Topic: Align to Reference Sequence
Replies: 8
Views: 66119

Align to Reference Sequence

I am assembling genomic resequencing data for a specific gene and would like to align to reference so that I can use a single reference file for all exons and have each assembly (by assay name) be the size of the amplicon rather than the huge open reading frame genomic sequence. I can achieve this b...